Porteur: Correa Cristian
Co-porteur(s): Simon Blanchet, Julien Julien Cucherousset, Delphine Vanhaecke, Pablo Saenz
Disciplines
Biologie intégrative : des gènes et des génomes aux systèmes
Biologie environnementale, écologie et évolution
Mots-clés
Biodiversity
metabarcoding
biological survey
geospatial biodiversity data
Chile
Exploradores River
fish distribution
Résumé
Biodiversity geospatial data is a key input for research and conservation planning, and monitoring plans using environmental DNA have promised standardized and cost-efficient methods to survey biodiversity in aquatic ecosystems. Previous studies by our team attempted cost-efficient vertebrate biodiversity surveys through water sampling and eDNA metabarcoding in c. 35 water bodies in Valle de Exploradores and neighboring water systems. We employed three different eDNA collection methods and sample processing procedures, involving labs and equipment providers from both France and Chile. While some of our results demonstrated the feasibility of this approach revealing reasonable vertebrate distribution patterns (fish, mammals, amphibian, and birds), we variously experienced challenges and drawbacks that ultimately caused partial or total failure to recover valid biodiversity data in some of our samples. The goal of this proposal is to enhance the bioinformatic pipelines to maximize geospatial eDNA-based biodiversity information, and critically assess the factors compromising the success of eDNA surveys practiced in remote locations. This might entail a few additional laboratory analyses of existing samples. Once cured, the eDNA-based fish biodiversity data will be compared to, and combined with, our fish occurrence observations (through various fishing methods) in search for inconsistencies and novelty. Finally, the integrated dataset will be added to the "Ichthyogeographic Atlas of Western Patagonia", an ongoing non-profit initiative funded by The Pew Charitable Trusts that have consolidated all freshwater and diadromous fish geospatial information available. This will allow a deeper understanding of the Bahia Exploradores biodiversity knowledge in a broader context.
Participants
CORREA CRISTIAN, Cucherousset Julien, Blanchet Simon, Saenz Pablo, Vanhaecke Delphine
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