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Polluants Métalliques, Intégrité Epithéliale et Résilience de la biodiversité animale du littoral méditerrannéen. (PoMIER)
Emmanuelle Renard
Session
2019
Co-leader(s)
Julien Issartel, Bernard Angeletti, Lars-Eric Heimburger
Project type
Projet OHM
Biologie, Écologie, Génétique, Océanographie
Les épithéliums sont des couches de cellules jointives qui assurent le contrôle de fonctions vitales en régulant les échanges milieu externe/milieu interne. Par conséquent, une perte d’intégrité de ces structures clés se traduit par différentes pathologies (ex: cancers). Ces épithéliums en tant que premières barrières de protection contre les agressions du milieu extérieur sont donc particulièrement exposées aux polluants présents dans l’environnement. L’impact de certains polluants métalliques sur l’intégrité des épithéliums de vertébrés a été montrée mais elle reste à évaluer chez d'autres animaux. Les épithéliums étant composés de protéines communes à tous les animaux (des éponges à l'homme), on peut craindre que certains polluants affectent également l'intégrité des épithéliums de nombreux animaux et soient une des causes de l'érosion de la diversité animale. Notre projet propose d'évaluer l'impact du mercure et du lithium, un contaminant émergeant du fait de l'usage croissant des batteries, sur l'intégrité et les capacités de régénération d'éponges, animaux filtreurs jouant un rôle clé dans les écosystèmes marins du littoral méditerranéen.

Leader

Emmanuelle Renard
E. RENARD is assistant professor at Aix-Marseille University (AMU) since 2001. She is coauthor of 20 publications in ACLI journals (hI 14), 2 online encyclopedia chapters, 3 protocols, 1 vulgarisation paper in french and 20 communications in international congresses (+2 papers in revision, 1 chapter submitted, 1 chapter in prep.) . Her domain, eco-evo-devo, is intrinsically a multidisciplinary domain, thus she is used to deal with complementary approaches. She has positive experience in management : head of a molecular facility lab with 3 technicians, head of 3 teaching units, coordination of a PICS project grouping 3 French and 1 Australian labs, co-supervision of >20 undergraduate internship projects, 5 master 2 interships and of 3 PhD projects (+ member of 3 PhD committees). Her scientific commitment was recognized by her university and by CNRS by the attribution of a bonus for scientific excellence (PES) and of two years of “teaching sabbatical” (delegation CNRS) to develop a project of the role of the notch pathway in the control of sponge epithelia involving both IMBE and IBDM labs. Her expertise is now recognized (e.g. invited to write 3 book chapters before spring 2019, co-organizer of conference session at the EFOR national conference (2016, 2017, 2019) and co-organizer of an international workshop (2015). (list of publication in the full CV)

Participants

Julien
Issartel
Julien ISSARTEL is assistant professor in ecophysiology of aquatic ectotherms (vertebrates and invertebrates) at Aix-Marseille University (AMU). He studies a variety of physiological mechanisms performed by aquatic animals when facing natural or anthropic constraints such as extreme temperatures, anoxia and metals (publications in integrative physiology, ecotoxicology and general ecology/science peer-reviewed journals). In 2010, he obtained an assistant professor (MCF) position at the Mediterranean Institute of Biodiversity and Ecology (IMBE; Aix-Marseille University). Julien Issartel's work is now focused on the impact of xenobiotics on essential physiological processes of aquatic ectotherms such as oxidative balance, energetic metabolism and osmoregulation.

Dayras, P(…)., Issartel, J., 2017. Osmoregulatory responses to cadmium in reference and historically metal contaminated Gammarusfossarum (Crustacea, Amphipoda) populations. Chemosphere 180, 412-422.
Auffan, M., (…),...
Carole
Borchiellini
C. Borchiellini has a strong background in Metazoan phylogeny, evo-devo and developmental regulator genes. She has a long-standing experience in using O. lobularis as a model. PhD director (6 PhDs), team leader (for 9 years) and project coordinator(1). Her capability as a scientific manager was recognized by the accreditation to supervise research (HDR) she obtained in 2007 and the quality of her scientific activity by the obtaining of a distinction (PES) by Aix-Marseille university in 2010 and 2017. In 2016 she obtains a promotion by the CNU. she has supervised or co-supervised 10 master 2 and 6 PhD.She is coauthor of 37 publications in international journals, 1 online encyclopedia chapters, 3 protocols and 71 communications in international congresses.

2018- Emmanuelle Renard (…) Carole Borchiellini. Understanding Animal Evolution: The Added Value of Sponge Transcriptomics and Genomics. BioEssay, doi : 10.1002/bies.201700237.
2018- Belahbib, H., (…) Borchiellini, C., &...
caroline
Rocher
Caroline Rocher is the lab manager of the molecular biology staff of the IMBE. She masters immunofluorescence and regeneration experiments on oscarella, her technical expertize will be helpful for this project.
Marjorie
Selva
marjorie Selva is technician in the molecular biology staff of the IMBE. She masters qPCR experiments planed in this project and will train the M2 student.
Kassandra
de pao
student in master 2 oceanography, she realizes her internship in the IMBE lab, under supervision of Julien issartel and Emmanuelle Renard. Her M2 project is dedicated to the present project. she aims at following a carrier as researcher in ecotoxicology.
Lars-Eric
Heimburger
Lars-Eric is CR 1 CNRS. His main interest is the application of novel techniques to outstanding questions on trace metal cycling in the ocean, with an emphasis on isotope, low-level speciation, and sensor approaches to understand marine mercury dynamics. He has been co-coordinator of 13 projects from 2011 to 2017 . he co-authored 30 publications in international journals (+ 3 submitted recently).

Outridge, P. M., […] & Heimbürger-Boavida, L. E. Updated Global and Oceanic Mercury Budgets […]. Environ. Sci. Technol. 52, 11466–11477 (2018). doi :10.1021/acs.est.8b01246
M Enrico, G Le Roux, LE Heimbürger, et al.(2017). Holocene atmospheric mercury levels reconstructed from peat mercury stable isotopes. Environmental Science & Technology 51 (3): 5899-5906 doi: 10.1021/acs.est.6b04238
X Fu, N Marusczak, LE Heimbürger, et al. (2016). Atmospheric mercury speciation dynamics at the high-altitude Pic du Midi Observatory, southern France. Atmospheric Chemistry and Physics 16(9):...
Aurélie
Dufour
AI CNRS. lab manager with expertise in environmental chemistry. ICP-MS and Hg analysis specialist.
Bernard
Angeletti
ingénieur d'étude en analyses chimiques. responsable du service d’analyses chimiques élémentaires par LA-ICP-MS et ICP-AES ; Gestion financière du service : Budjet annuel de fonctionnement de 50 KЄ. Tarification. Gestion contrats industriels ; Développement instrumental et méthodologique. Contrôle qualité ;Analyses en phase liquide d’éléments traces ou ultra-traces par ICP-MS, Perkin Elmer NexIon 300X, ou d’éléments majeurs et mineurs par ICP-AES, Perkin Elmer Optima, 4300DV ;Analyses par couplage microscope d’Ablation Laser, CETAC LX 213 G2+, ICP-MS. Réalisation de cartographies élémentaires semi-quantitatives.Compétences : pétrochimie, radioprotection, analyses chimiques, hygiène et sécurité.

Veronique Lenoble, (...), Bernard Angeletti , et al.; Nickel retention by an ion-imprinted polymer: Wide-range selectivity study and modelling of the binding structures;The Chemical Engineering; Journal 304 • June 2016
Paul, E., (...), E., Angeletti, B.,et...
Amélie
vernale
doctorante 2e année en co-tutelle IBDM/IMBE. en seconde année elle développe un projet sur la transition épithélio-mésenchymateuse chez oscarella. elle aidera au suivi des processus cellulaires au cours de la régénération sur lesquelles elle a déjà réalisé des manipulations. elle est 1er auteur d'un poster, co-auteur d'un article et d'un chapitre en préparation
André
Le Bivic
DRCE CNRS since 2017
Since 2012: Director of IBDM (Marseille Institute for developmental biology, UMR 7288)
Since 2009: In charge of the Young group leader program of CNRS-Inserm (ATIP-Avenir)
Since 1997: Group leader.
2011: Deputy Director of Life Sciences at CNRS (INSB)
His group is specialized in the study of epithelial polarity and morphogenesis and is working on the role of polarity complexes in the establishment of the apical pole of epithelial cells and in the formation and maintenance of epithelial junctions (TJs and AJs). We master cell biology approaches on epithelial cells in vitro (Caco2) and in vivo (Drosophila) and with the imaging facility of IBDM (FBI) we are using several approaches (confocal, live imaging, STED and PALM) to localize and analyze the dynamics of apical and junction proteins. Partner in 2 EU networks on Crumbs, CRUMBS Therapeutics and CRUMBS Insight, coordinator of an ANR Crumbs in 2007).

Toret, C. &A. Le Bivic. 2018. A potential Rho GEF and...